Journal: Scientific Reports

Article Title: Transgenic Eimeria tenella as a vaccine vehicle: expressing TgSAG1 elicits protective immunity against Toxoplasma gondii infections in chickens and mice

doi: 10.1038/srep29379

Figure Lengend Snippet: The levels of IgG 1 and IgG 2a antibodies to TgSAG1 2 weeks after the boost immunization were analysed via ELISA with recombinant TgSAG1 (n = 10).

Article Snippet: The ELISA protocols were conducted as well as in the above description, except the secondary antibodies used in these assays were an HRP-conjugated goat anti-mouse IgG (Proteintech Group Inc., Chicago) and an HRP-conjugated goat anti-mouse IgG 1 or IgG 2a (Proteintech Group Inc., Chicago) for the isotype analyses.

Techniques: Enzyme-linked Immunosorbent Assay, Recombinant

Journal: bioRxiv

Article Title: Hypoimmunogenic human motor neurons induced from iPSCs in vivo substantially ameliorate ALS disease in large animal models

doi: 10.1101/2025.09.03.673895

Figure Lengend Snippet: (A) Schematic illustration of multiplex genetic-editing strategies for engineered HIP-NILB-iPSCs. (B) Indel analysis by Sanger sequencing for B2M , CIITA , IL6ST and TNFRSF1A in HIP-NILB-iPSCs. (C) Real-time PCR analysis of CD47 , CD24 , IgG1-Fc , CTLA4 and PD-L1 in HIP-NILB-iPSCs. (D) Western blot analysis and relative density quantification of B2M, CIITA, IL6ST, TNFR1, CTLA4-Ig, PD-L1, CD24 and CD47 in HIP-NILB-iPSCs of three independent samples. CIITA was detected in NILB-iPSCs and HIP-NILB-iPSCs upon IFN-γ and TNF-α treatment for 48 h. (E) Karyotyping of HIP-NILB-iPSCs. (F) Scatter plot comparison of transcriptomes for HIP-NILB-iPSCs and NILB-iPSCs. The raw counts for each gene were transformed to log10 value, and genes with more than 1 count in each sample were shown. The pluripotent-related genes were labeled in the diagram. Dashed lines depict the 10-fold changes. The R 2 was determined by Pearson’s correlation. The NILB-iPSCs were used as a control. (G) Heatmap showing expression level of stem cells-related and neuron development-related genes together with the enrichment analysis in WT iPSCs and HIP-NILB-iPSCs upon Dox induced for 0.5-, 4- and 7-days. Data are mean ± SEM; p values were determined using a two-tailed, unpaired Student’s t-test ; * p < 0.05; ** p < 0.01.

Article Snippet: The antibodies used were listed as follows: PE anti-human HLA-A,B,C (BioLegend, 311405, 1:20), PE anti-human HLA-DR,DP,DQ (BioLegend, 361716, 1:20), PE Mouse IgG2a, κ Isotype Ctrl (FC) antibody (BioLegend, 400213, 1:20), PE anti-human CD3 (BD Biosciences, 347347,1:50), FITC anti-human CD107a (BD Biosciences,555800,1:100), FITC Mouse IgG1, κ Isotype Control RUO (BD Biosciences, 555748,1:100), APC anti-human CD56 (BioLegend, 362504, 1:20), PE-Cy5 human CD56 (BD Biosciences, 555517, 1:10), APC Mouse IgG1, κ Isotype Ctrl (FC) antibody (BioLegend, 400122, 1:20), PE anti-mouse CD68 (BioLegend, 137014, 1:100), PE Rat IgG2a, κ Isotype Ctrl (BioLegend, 400508, 1:100), CoraLite® Plus 647-conjugated IBA1 monoclonal antibody (Proteintech, CL647-66827,1:200), CoraLite® Plus 647 Mouse IgG2a Isotype Control (Proteintech, CL647-65244,1:200).

Techniques: Multiplex Assay, Sequencing, Real-time Polymerase Chain Reaction, Western Blot, Comparison, Transformation Assay, Labeling, Control, Expressing, Two Tailed Test

Journal: Frontiers in Immunology

Article Title: Toll-Like Receptor 4 on both Myeloid Cells and Dendritic Cells Is Required for Systemic Inflammation and Organ Damage after Hemorrhagic Shock with Tissue Trauma in Mice

doi: 10.3389/fimmu.2017.01672

Figure Lengend Snippet: Toll-like receptor 4 (TLR4) drives systemic inflammation and end-organ damage in a mouse model of hemorrhagic shock (HS) and trauma (HS/T). Wild-type (WT), TLR4 −/− , MyD88 −/− , or Trif −/− B6 mice were subjected to HS/T. IL-6 and ALT plasma levels were measured 6 h after the onset of HS/T as described in Section “ ” (A,B) . In separate experiments, the selective anti-mouse TLR4 Ab 1A6 or its isotype control (W6/32 Ab) was administered intravenously 30 min prior to the initiation of the HS and the levels of IL-6 and ALT were measured at 3, 6, and 20 h after the onset of HS/T (C,D) . Panels (A,C) show IL-6 plasma levels, (B,D) show ALT plasma levels, (E,F) show IL-6 and ALT plasma levels after 6 h following the initiation of HS/T in the following cell-specific TLR4 −/− B6 mouse strains: Albumin-Cre × TLR4 loxP/loxP (Alb-Cre), CD11c-Cre × TLR4 loxP/loxP (CD11c-Cre), and Lyz-Cre × TLR4 loxP/loxP (Lyz-Cre). WT and TLR4 loxP/loxP (FloxP) mice were used as controls. Panels (G,H) show IL-6 and ALT plasma levels in lethally irradiated WT B6 mice reconstituted with bone marrow cells from CD11c-diphtheria toxin (DT) receptor (DTR) B6 mice or CD11c-TLR4 −/− B6 mice at 6 h after HS/T. WT B6 mice were used as controls [ (A,B,E,F) * P < 0.05, analyzed by Mann–Whitney Rank Sum Test; (C) P = 0.050, (D) * P < 0.001, analyzed by two-way ANOVA; (G,H) * P < 0.001, analyzed by Student’s t -test ( n = 6–15 animals/experimental condition)].

Article Snippet: Anti-mouse TLR4 monoclonal antibody (mAb), 1A6, and its isotype-matched control mAb, W6/32, were provided by NovImmune SA (Geneva, Switzerland) and have been described previously ( ).

Techniques: Irradiation, MANN-WHITNEY